Description
Field Application Guide
1. Target Trees
- Species: Aquilaria malaccensis
- Minimum Age: ≥5 years
- Minimum DBH: 10 cm (4 inches)
2. Application Overview
BarIno™ FusaPrime™ is a liquid fungal inoculant designed to initiate natural agarwood resin formation.
- Primary function: Activate resin biosynthesis in wounded xylem
- Secondary use: Baseline phase for sequential induction system (follow-ups: FusaTrinity™)
3. Required Materials
- BarIno™ FusaPrime™ (500 mL, 1 L, or 5 L bottles)
- Drill or hand auger
- Biodegradable plugs or wax sealant
- Measuring cylinder or syringe for accurate dosing
- PPE: gloves, mask, eye protection
4. Pre-Application Preparation
- Inspect Tree Health: Avoid diseased, stressed, or young trees.
- Calculate DBH: Measure diameter at breast height (1.3 m from ground).
- Determine Inoculation Points:
- Trees 10–20 cm DBH → 1–2 holes
- Trees 21–40 cm DBH → 3–4 holes
- Trees >40 cm DBH → 5–8 holes
- Prepare Inoculant: Shake bottle gently to ensure uniform suspension
5. Drilling & Injection Procedure
- Step A – Drill Holes
- Drill at a 45° angle to trunk surface
- Hole depth: 3–5 cm or 2–3 times the tree radius for larger DBH
- Hole diameter: 8–10 mm
- Space holes evenly around the trunk for optimal resin distribution
- Step B – Inject Inoculant
- Use sterile syringe or pipette
- Inject 10–50 mL per hole (adjust according to DBH)
- Avoid overflow or spillage
- Step C – Seal Holes
- Insert biodegradable plug or wax seal
- Prevents contamination and fungal competition
6. Post-Application Monitoring
- Inspect inoculation points monthly
- Look for:
- Resin exudation (“resin beads”)
- Healthy callus formation around hole
- Record resin volume, color, and texture for traceability
7. Sequential Induction
- After 3–6 months, apply secondary induction products (FusaTrinity™) to increase resin yield and density
- Follow the same drilling/injection/sealing procedure
8. Safety Guidelines
- Wear PPE (gloves, mask, eye protection)
- Avoid inhalation of aerosols
- Wash hands thoroughly after handling inoculant
- Dispose of unused inoculant and contaminated materials per biohazard regulations
9. Key Tips for Maximum Resin Yield
- Tree Health is Critical: Only apply to healthy, actively growing trees
- DBH-Based Dosing: Prevent under- or over-inoculation
- Even Distribution: Ensure resin initiation points are spread around the trunk
- Follow Sequential Induction: Combine with FusaTrinity™ & FusaBlaze™ for export-grade oud oil
- Record-Keeping: Maintain a digital log or field sheet to track resin initiation and yield
