To pre-condition Aquilaria trees for enhanced resin induction using a controlled biochemical primer, reducing damage while improving resin yield and uniformity.
1. Target Trees
- Species: Aquilaria malaccensis
- Age: ≥ 4 years
- DBH (Diameter at Breast Height): ≥ 8 cm
2. Materials Required
- BarIno™ AgarStart™ solution
- Hand drill or mechanical drill
- Injection syringes or peristaltic pump
- Tree plugs or parafilm (to seal drilled holes)
- Gloves and safety goggles
3. Pre-Application Preparation
- Select healthy, actively growing trees.
- Inspect for disease or structural weakness; avoid stressed or sick trees.
- Prepare the solution: ready-to-use from the manufacturer; shake gently before use.
- Wear PPE (gloves, goggles) during handling and injection.
4. Drilling & Injection Protocol
- A. Hole Placement
- Spiral pattern around the trunk starting ~1 m above ground
- Maintain 10–15 cm spacing between holes
- B. Drilling Parameters
- Depth: 5–7 cm
- Angle: 30°–45° to the trunk surface
- Hole diameter: 8–12 mm (adjust per drill bit availability)
- C. Injection
- Inject 10–15 mL of BarIno™ AgarStart™ per hole
- Insert syringe tip fully and dispense slowly to avoid overflow
- D. Hole Sealing
- Seal each drilled hole with a plug or parafilm to prevent contamination and moisture loss
5. Timing & Environmental Considerations
- Apply during early morning or late afternoon to reduce heat stress
- Avoid application during heavy rain or drought conditions
- Ideal temperature range: 20–30°C
6. Post-Application Monitoring
- Monitor trees for early biochemical response (internal darkening, minor sap exudation)
- Maintain plantation health: adequate water and nutrition
- Plan follow-up induction steps using BarIno™ FusaPrime™ after 30–60 days
7. Safety & Handling
- Avoid direct skin or eye contact
- Wash hands thoroughly after application
- Keep away from children and livestock
- Dispose of any unused product according to local environmental regulations
8. Expected Results
| Timeframe | Observed Response |
|---|---|
| 0–1 month | Activation of defense pathways, early phenolic buildup |
| 1–2 months | Micro-resin formation, tissue priming |
| 3–6 months | Early resin accumulation, ready for next-stage microbial/fungal induction |
| 6–12 months | Resin maturation, high-grade agarwood formation |
9. Tips for Optimal Results
- Ensure even hole distribution around the trunk
- Avoid over-injection to prevent tissue stress
- Combine with nutrient and irrigation management for best outcomes
- Record each tree’s application date for monitoring and sequential induction scheduling