Description
Field Application Guide
Microbial Enzyme-Based Agarwood Inducant
1. Recommended Trees
- Species: Aquilaria malaccensis
- Age: ≥3 years old
- Diameter at Breast Height (DBH): ≥10 cm
- Health: Healthy, structurally sound trees without major infections
2. Site Preparation
- Clear debris around the base and trunk.
- Ensure good drainage and avoid waterlogged areas.
- Map inoculation points along the trunk and main branches
3. Wounding / Drilling
- Hole Diameter: 10–15 mm
- Depth: 3–5 cm into xylem
- Angle: Slightly upward (~45°) to prevent water accumulation
- Spacing: Every 30–50 cm along the trunk depending on DBH
- Sanitation: Sterilize drill bits or knives before each tree to prevent contamination
4. Inoculant Preparation
- Dilution: 1:10 with sterile or clean water (adjust according to TDS)
- Mixing: Gently invert; avoid shaking that could damage microbial cells
- Storage: Keep shaded and cool; use immediately after preparation
5. Application
- Injection:
- Use sterilized syringes, injector bottles, or pipettes.
- Deliver 5–10 mL per wound.
- Optional Sealing:
- Apply organic sealant (beeswax or biodegradable paste) to retain inoculant and prevent contamination.
- Record-Keeping:
- Log tree ID, DBH, wound locations, inoculant batch number, and application date.
6. Post-Application Care
- Monitor for contamination or pest attacks.
- Maintain optimal soil moisture (avoid overwatering or drought stress).
- Optional follow-up application at 2–3 months if initial resin induction is low.
7. Observation & Resin Harvest
- First Signs of Resin: 4–6 months post-inoculation
- Full Resin Formation: 6–8 months depending on tree age and environment
- Harvesting:
- Collect resinous chips carefully to avoid damaging the tree.
- Maintain traceability for quality control and export compliance.
8. Safety Guidelines
- Wear gloves, long sleeves, and eye protection.
- Avoid direct inhalation or ingestion.
- Clean and disinfect all equipment after use.
9. Optional Enhancements
- Sequential Induction Protocol: Use with BarIno™ Sequential System for mature trees to maximize yield.
- Supportive Care: Organic foliar sprays or soil probiotics to enhance overall tree health and metabolism.
